western blotting

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bugme
bugme's picture
western blotting

I am going with pierce... thank all!

jay
jay's picture
pierce

pierce

Spighetta
Spighetta's picture
Amersham cod. RPN2132

Amersham cod. RPN2132

got777
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biorad immunstar

biorad immunstar

bugme
bugme's picture
jay wrote:pierce

jay wrote:

pierce

Yes, Pierce is working good.

Fraser Moss
Fraser Moss's picture
Amersham (GE healthcare as

Amersham (GE healthcare as they now are) ECL kits

RH
RH's picture
amersham is the best.

amersham is the best.

samm
samm's picture
I have found the Pierce

I have found the Pierce SuperSignal West Pico Chemiluminescent kit with stablized peroxide to work very well - my experience with the Pierce product has been better than with Amersham.

ksettle
ksettle's picture
You can also detect mRNA

You can also detect mRNA presence/production with a branched-DNA (dBNA) detection probe set for the protein of interest. Results are directly amplified from the mRNA in cell lysate, and yield a chemiluminescent signal that is quantifiable with any light-sensitive reader. (May require a custom probe set if none for your target protein are commercially available).

Richard Taylor
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Have you looked into the Li

Have you looked into the Li-Cor Odyssey?

nin1318
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samm wrote:I have found the

samm wrote:

I have found the Pierce SuperSignal West Pico Chemiluminescent kit with stablized peroxide to work very well - my experience with the Pierce product has been better than with Amersham.

i have used both amersham and pierce...currently use amersham. have had good results with both.

badcell
badcell's picture
I too have tried both Pierce

I too have tried both Pierce and Amersham kits. I don't really like Pierce, though. Amersam ECL and ELC Plus work better in my hands.

camembert
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did pierce satisfy I might

did pierce satisfy I might use it in the future

bugme
bugme's picture
camembert wrote:did pierce

camembert wrote:

did pierce satisfy I might use it in the future

pierce did a very good job. used amersham bioscience nitrocellulose though.

MadScientist74
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I use Pierce... but a batch

I use Pierce... but a batch crapped up on me due to production fault... then I tried Santa Cruz... it was good.

Now back to Pierce....

Definitely beats Amersham in terms of stability and luminescence.

My 2 cts.

YB
YB's picture
don't know the Pierce one....

don't know the Pierce one....

Currently using Roche : also definitely beats Amersham in terms of stability and luminescence. ;-)

mchinmoyee
mchinmoyee's picture
I mostly used Amersham ECL,

I mostly used Amersham ECL, to increase sensitivity or to detect very low amount of protein present one should go for Advance ECL from Amersham.

kelly26lima
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Me too, are you using west

Me too, are you using west pico supersignal ? DOes it work well?