I am going with pierce... thank all!
Amersham cod. RPN2132
Yes, Pierce is working good.
Amersham (GE healthcare as they now are) ECL kits
amersham is the best.
I have found the Pierce SuperSignal West Pico Chemiluminescent kit with stablized peroxide to work very well - my experience with the Pierce product has been better than with Amersham.
You can also detect mRNA presence/production with a branched-DNA (dBNA) detection probe set for the protein of interest. Results are directly amplified from the mRNA in cell lysate, and yield a chemiluminescent signal that is quantifiable with any light-sensitive reader. (May require a custom probe set if none for your target protein are commercially available).
Have you looked into the Li-Cor Odyssey?
i have used both amersham and pierce...currently use amersham. have had good results with both.
I too have tried both Pierce and Amersham kits. I don't really like Pierce, though. Amersam ECL and ELC Plus work better in my hands.
did pierce satisfy I might use it in the future
pierce did a very good job. used amersham bioscience nitrocellulose though.
I use Pierce... but a batch crapped up on me due to production fault... then I tried Santa Cruz... it was good.
Now back to Pierce....
Definitely beats Amersham in terms of stability and luminescence.
My 2 cts.
don't know the Pierce one....
Currently using Roche : also definitely beats Amersham in terms of stability and luminescence. ;-)
I mostly used Amersham ECL, to increase sensitivity or to detect very low amount of protein present one should go for Advance ECL from Amersham.
Me too, are you using west pico supersignal ? DOes it work well?