I seem to be in a bit of a pickle and I was really hoping that somebody could help me please.
Recently we did an experiment wherewe had to determine the activity and kinetic parameters of a certain enzyme. For the second part, we had to draw a Michealis Menten plot. This is where I got a bit lost
I constructed graphs for Absorbance vs Time, and used their slopes in the Beer Lambert law in order to get concentration per time. From here I need touse these to plot a Michealis menten graph but I am not sure how. Should I use Activity (U/ml) , total activity (U) or specific activity (U/mg) to plot the graph?