Hi,
My problem is, I am not getting good resolution for Native PAGE. The protein is an enzyme, actually a cocktail of cellulase enzyme. I have tried it with 7%, 12% AND 15% gel concentration. 15% was better. But the higher molecular weight proteins are too dense plus this cocktail has too many proteins. I am using the Bio-RAD assembly and standard protocol for electrophoresis. what should I do?
Electrophoresis
29 August 2013
#1
NISHITA
Electrophoresis
Have you tried a gradient gel? (says 4-15% or 4-20%, Bio-rad does carry these)
Have you considered aapillary PAGE and activity staining?
Biju Joseph