Will somebody help me out about the about tris-cl & tris-HCL difference? Also, after plant DNA extraction do i need to add RNase after final resuspension of DNA pellete in TE buffer or autoclaved dis.water? How do I inactivate RNase after this step?
The electrophoresis buffer should be at pH 7.5- 8.so inorder to make tris base which is originally at pH 11 , we add HCl to it and thus it is made tris -HCl and that is all the difference. commercially available tris HCl are having molecular weight greater the tris base and pH will be 8.
Thanks so much Argerine, I was partially aware of that, the only thing I was confused about-if in protocol 1M Tris-HCL is mentioned, can I use tris-cl to make that 1M solution if it gives the same pH as tris-HCL.
No you can use both and consider same they will give different pH
thanks a lot....