Difference between tris cl & tris HCL?

5 posts / 0 new
Last post
poojapandya
poojapandya's picture
Difference between tris cl & tris HCL?

Will somebody help me out about the about tris-cl & tris-HCL difference? Also, after plant DNA extraction do i need to add RNase after final resuspension of DNA pellete in TE buffer or autoclaved dis.water? How do I inactivate RNase after this step?

g a
g a's picture
 Hi Pooja

 Hi Pooja

The electrophoresis buffer should be at pH 7.5- 8.so inorder to make tris base which is originally at pH 11 , we add HCl to it and thus it is made tris -HCl and that is all the difference. commercially available tris HCl are having molecular weight greater the tris base and pH will be 8.

poojapandya
poojapandya's picture
 Thanks so much Argerine, I

 Thanks so much Argerine, I was partially aware of that, the only thing I was confused about-if in protocol 1M Tris-HCL is mentioned, can I use tris-cl to make that 1M solution if it gives the same pH as tris-HCL.

g a
g a's picture
 No you can use both and

 No you can use both and consider same they will give different pH

poojapandya
poojapandya's picture
 thanks a lot....

 thanks a lot....