I am confused with a small experimental result.
I got there is different in mobility of immunoprecipitaed importin Beta with that of importin ateBeta from whole cell
lysate. i did ip with gfp ab. from GFP-fusion construct containing NLS motif transfected cell sample and their whole cell lysate followed by WB with Importin Beta. I found IP importin Beta detected at a higher shift than from whole cell lysate and I became confused.
Can you suggest me why it is happening so?
every time I got same results.
so, what would be the reseason for difference in mobility?
If you help me I will be grateful to you.