Please advise me the properly way to take out the DTT from bottle, since it is in powder form and easily oxidized.
DTT is stored at a lower temperature around 4C. Ensure that you are bringing the vial to room temperature prior to opening failing which will otherwise lead to condensation/ liquefication.
Try and open the vial for as short duration as you can.
First of all, thank you for your valuable information. By the way, i have some questions to ask for:
1. That's mean have to take out DTT from 4 C and left it at room temperature for a while (around 1 minute),
then only open it right?
2. When wanna to weight the DTT, any properly way to take out the powder DTT from bottle?
Let the vial reach ambient temperature and then only open it. Its a common practice for every salt/ solution stored at low temperature.
Thanks you for teaching and prompt replying. Really thanks.