I am working on the kinetics of the carrier protein binding to small molecular proteins and peptides..... Does any1 have experience in handling the uv spectroscopy or CD spectra..... Will it be useful for this kind of work..... I mean the spectra will show varaitions in binding if a pure protein is treated with binding peptides but in my case i have a fraction of proteins which is bound to the carrier protein but then......how can i study kinetics of protein interactions in this case ........... I have very little idea and Im relatively a beginner in the field....
we also dont want ot use detergents or urea or acetonitrile for the protein protein disruption work....... especially detergents......
Kindly help if you can
Thanking you in anticipation.
protein - protein interactions