protein quantification using Lowry method

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kipngetich
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protein quantification using Lowry method

Hi!
 I want to quantify my protein extracts  that have been solubilized in IEF sample buffer. what do i do?  can some one help.

Satinder
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 IEF sample buffer contains

 IEF sample buffer contains DTT , ampholytes, CHAPS which interfere with quantification using bradford's method. Reduce the concentration of these agents by diltuing to the compatible concentrations for the method of quantification you are goinf to use.

Try solubilizing protein first in a compatible buffer, quantify and then solubilize in the IEF buffer from next time onwards.

Chin Fen Teo
Chin Fen Teo's picture
 Kipngetich,

 Kipngetich,
I recommend BCA assay. Below is a link to the reagent (and procedure details).
http://www.piercenet.com/products/browse.cfm?fldID=02020101
Good luck.

kipngetich
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 Thanks let me try it.

 Thanks let me try it.

kipngetich
kipngetich's picture
 Thanks, it is wise to

 Thanks, it is wise to solubilize first in compatible buffer first then solubilze in the IEF buffer.