Amplex Red Cholesterol quantification

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Guy Sovak
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Amplex Red Cholesterol quantification

Any one did this assay?

Guy Sovak
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OK,

OK,
I did it on friday. Amplex red is a great tool. Did some testing for the ammpount of Cholesterol in 3T3 cell and it works great.
If any one needs help I am here.
Guy

Nipon
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Hi, I'm gonna use the Amplex

Hi, I'm gonna use the Amplex Red kit to quantify the cholesterol content of Cell lysates. I was wondering if the Reaction buffer of the kit is sufficient as lysis buffer ( 0.5M potassium phosphate, pH7.4, 0.25M NaCl, 25 mM cholic acid, 0.5% Triton X-100).
I found lots a publication with different lysis buffer, but some a similar content than the reaction buffer.

Thanks
Nipon