ADP solution

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Taiyou
Taiyou's picture
ADP solution

hi everybody,

In my experiment I have to prepare some reagents that have not been contaminated by ATP. I need the pure ADP solution, therefore, I used Lr/L reagent in the ATP assay (Promega) to check the ATP contamination in both of water and ADP solution.
I used the Luminometer to measure the presence of ATP in ADP solution. The control is only water. Unfortunately, the value got from the sample containing ADP solution is very high comparing to the control. I don’t know why the ADP solution containing ATP? because the water used as the solvent of ADP was not contaminated by ATP. And the ADP solution making condition is not ATP-contaminated.
Could any one help me explain why in the ADP solution (using water as a solvent), the ATP component is appeared?

Thank a lot!
 

Monu
Monu's picture
 hi taiyou, I will suggest

 hi taiyou, I will suggest you to check the ATP contamination using some other assay kit. Even after that if it comes, then I will suggest you to use HPLC grade water for your experiments.

Taiyou
Taiyou's picture
Dear Monu,

Dear Monu,
Thank you for any your suggestion! which assay kit do you suggest me to check ATP? please show me some more information!
With best regards!
Taiyou