Triton?

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leelotau
leelotau's picture
Triton?

What is triton?
What are the effect of triton on the activities of lactase dehydrogenase, malate dehydrogenase and succinate dehydrogenase

heehawmcduff
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Triton X-100 is a relatively

Triton X-100 is a relatively mild non-denaturing detergent which, in terms of cell biology at least, can be used at low concentrations to provide a solution which will cause cell lysis.  I'm not sure about the effects of TritonX on the latter two enzymes that you mention, but I have commonly used TritonX as a positive control to lyse cells when quantifying LDH release and it does not appear to interfere with the detection of LDH at all.  Biological effects may be another story...

http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/t8532pis.Par.0001.File.tmp/t8532pis.pdf

g a
g a's picture
Hi leelotau

Hi leelotau

Any detergent will disrupt the tertiary structure of a protein interfering with hydrophobic interactions amongst the aminoacids.

Triton X-100 is nonionic and is relatively weaker detergent as compared to SDS or other like CHAPS etc.

according to this link a milder conc of triton used over here is for release of LDH.

http://www.chembiotek.com/ChemBioTek-HTML/datasheet/InVitroADME/LDH.pdf

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WXH-4DD2SDS-8&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=841ccafa5389358f1d1b05cbcdf9d8c6