I am working on transcriptomics using next-generation sequencing. I have extracted total RNA from trypanosomes and checked the purity of the total RNA using Bioanalyser and have sent the samples for sequencing. When the sequencing result is back I will be looking at genes that are up- or down-regulated. The problem is that I don't know how to begin to analyse the data that will be generated to enable me see genes that are up-regulated or down-regulated. I learnt that a huge amount of data will be generated and how do I begin the bioinformatic analysis to arrive at the answer. Can anyone help please?