need some hints

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Nazunderwent
Nazunderwent's picture
need some hints

hello All
i just wonder how could i get the sequence of active sites of enzyme
any suggession are very wellcome
cheers

Sami Tuomivaara
Sami Tuomivaara's picture
Nazunderwent,

Nazunderwent,

All active site prediction algorithms need at least the protein sequence, some need full 3d structure.

There are some online prediction sites available, here's just two:
http://www.scfbio-iitd.res.in/dock/ActiveSite.jsp
bioinformatics.cm-uj.krakow.pl/activesite/

You can also google "enzyme active site prediction" and you get links to published literature, there is lot of it available.

Cheers,

Nazunderwent
Nazunderwent's picture
Thanks for your help

Thanks for your help
but one thing more
now i am trying to purify a cytosolic protein which might have enzymatic activity
the qustion is
how could i start that 
could you explain for me in brief ?
the protein is hypothetical i have the amino acid sequence but i do not know where is it in the genome of the bacteria ?
how could i find it in the e.g NCBI ?
what steps shall i follow ?
Cheers