protein purification

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Nazunderwent
Nazunderwent's picture
protein purification

a cytosolic protein does not have signal peptidase cleavage site according to signal peptide prediction software
the protein is hypothitcal from bacterial sp.
i want to clon this protein and purify it
and seek some help from you
transmembrane domains does not exsist
how that could affect my experiment and how difficult the purification is ?
is there are any possible ways to purify it in full native structure without any distoration ?
please where is the bioinformatician and protein experts in this field ? answer me please