Agarose gel electrophoresis

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Emma Elliot
Emma Elliot's picture
Agarose gel electrophoresis

My ladder is 100bp.I have two primers to test out. Primer Y which amplifies up to a product of 200bp and primer Z which amplifies up to 150bp fragment.I'm not very sure of how this all works. What does this mean? I thought that the ladder has to be bigger than the size of the primer. How can you measure with 100bp ladder.

Guy Sovak
Guy Sovak's picture

YesYou need to use a bigger ladder 1K will do it.