I made some cross-linked gelatin gels and intended to use these for cell culture. To my surprise, cells like NIH3T3 or BHK 21 dont attach/spread on to these gels readily. I thought gelatin is frequently used for coating plastic culture surface to promote cell attachment.
These are glutaldehyde fixed gelatin 5% (w/v), extensively washed after blocking the reactive groups with glycine. Cells are not spreading on it after two days, most remain rounded, while they are very happy on the regular plastic dishes.
Anyone has some comments on whats going on here?
Thanks in advance!