I'm using 4% stacking and varying from 7.5% to 11% separating gels (Tris-HCl). I'm getting decent resolution but the dye front is extremely wonky.. I don't really understand why. My first reaction was that it was a leak, but i put the unit back together multiple times the first time it happened and it continued to occur. I've had this problem numerous times now, I've checked for leaks every single time from the inner chamber but they definitely don't exist. So anyone have any ideas? Major thanks, I'm feeling pretty stupid about this.
Edit: Oh, and I've tried a variety of different voltages, I usually just use 100V though.
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