Wound-healing assays for cell migration studies

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omid
omid's picture
Wound-healing assays for cell migration studies

Is there a method to study wound-healing using electrical field?. I am looking for an automated instrument to monitor cell behavior in tissue culture, I think the instrument is called ECIS. Does anybody know the name of the related products and companies?

vanishing
vanishing's picture
I found this publication:

I found this publication:

Keese et al., 2003
PNAS | February 10, 2004 | vol. 101 | no. 6 | 1554-1559
Electrical wound-healing assay for cells in vitro

http://www.pnas.org/cgi/content/full/101/6/1554

They use a ECIS Model 100 or Model 1600R from Applied BioPhysics

http://www.biophysics.com/

BioPhysics
BioPhysics's picture
Hello.

Hello.

I was doing a search on ECIS stem cells and I came across your question. I actually work for Applied BioPhysics, we invented and manufacture the ECIS system.

I realize this post is almost 2 years old, perhaps you are still conducting wound healing assays. If so, please feel free to contact me for more information. eval(unescape('%64%6f%63%75%6d%65%6e%74%2e%77%72%69%74%65%28%27%3c%61%20%68%72%65%66%3d%22%6d%61%69%6c%74%6f%3a%67%6f%6f%64%62%72%65%64%40%62%69%6f%70%68%79%73%69%63%73%2e%63%6f%6d%22%3e%67%6f%6f%64%62%72%65%64%40%62%69%6f%70%68%79%73%69%63%73%2e%63%6f%6d%3c%2f%61%3e%27%29%3b'))

In a nut shell our systems uses a weak AC signal, we use impedance to measure the cells that settle on the base of a well. Once the cells have reached confluence, we can increase the current, the cells on the 250 micrometer electrode can be electroporated and killed. In realtime you see a drop in the impedance (open electrode values), you can then monitor the healthy cells around the electrode migrate in to "heal" the wound. I have attached a comprehensive, peer reviewed publication for your review.

All the best to you and your research -
Jenn

minniegupta
minniegupta's picture
Hi,

Hi,

Not sure if you are still checking for responses to your initial question, but Platypus Technologies offers a cell migration assay that is reproducible and pretty easy to use. It has an advantage over the ECIS system in that you don't have to purchase an expensive piece of equipment. You can simply use a fluorescence platereader. Check www.platypustech.com/discoverAssay.html

Good luck!

Minnie Gupta