Need help to select BD Biosciences products for migration & invasion assay

3 posts / 0 new
Last post
Hui Hui
Hui Hui's picture
Need help to select BD Biosciences products for migration & invasion assay

v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}

Normal
0

false
false
false

MicrosoftInternetExplorer4

/* Style Definitions */
table.MsoNormalTable
{mso-style-name:"Table Normal";
mso-tstyle-rowband-size:0;
mso-tstyle-colband-size:0;
mso-style-noshow:yes;
mso-style-parent:"";
mso-padding-alt:0in 5.4pt 0in 5.4pt;
mso-para-margin:0in;
mso-para-margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:10.0pt;
font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";
mso-ansi-language:#0400;
mso-fareast-language:#0400;
mso-bidi-language:#0400;}

v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}

Normal
0

false
false
false

MicrosoftInternetExplorer4

/* Style Definitions */
table.MsoNormalTable
{mso-style-name:"Table Normal";
mso-tstyle-rowband-size:0;
mso-tstyle-colband-size:0;
mso-style-noshow:yes;
mso-style-parent:"";
mso-padding-alt:0in 5.4pt 0in 5.4pt;
mso-para-margin:0in;
mso-para-margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:10.0pt;
font-family:"Times New Roman";
mso-fareast-font-family:"Times New Roman";
mso-ansi-language:#0400;
mso-fareast-language:#0400;
mso-bidi-language:#0400;}

 
Hi everyone,

I would like to perform tumor cell migration and invasion assay after tumor cells transfected with miRNA for my master study. I like to purchase migration and invasion assay kit from BD Biosciences but somehow the website is so confusing to me that make me chouldn't select and purchase their products. Anyone did this assay before and using BD Biosciences products before? In BD website, they are BD Biocoat, BD falcon and BD falcon fluoroBlok, which one should i select since my lab do not have any fluorescent microscope or fluorescent detector and i just like to do manual count under light microscope after cells staining. Btw, im going to work on 2 cell lines only which do not have a lot samples, so should i purchase individual cell culture insert or multiwell insert system? I am so confusing with this website

. what is companion TC plates? Feeder tray? Should i purchase? Which product should i buy for migration assay? and also which product for invasion assay?

Thanks a lot!!!!

-Hui Hui-

catwalion
catwalion's picture
Hi,

Hi,

sorry it's not an answer to the previous question, I was wondering about this type of test, since I use BD Biosciences adhesive test twice but there wasn't any reproductivity and the result was a bit odd. did someone have any idea to have a better result?
For invasion instead of the BD bioscience kit I purchased the porous membranes and the different compound to add to well it's was cheaper, but still for the result you need a fluorescent detector to read calcein. and the result was still bad.
in contrast for migration you can use a simple test wound healing (scratch test)

Biju
Biju's picture
The best to find out is call

The best to find out is call BD support and explain to them. They shoudl be able to guide you.
Biju