ROS/NOS

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b.curious
b.curious's picture
ROS/NOS

 I been trying to do Griess assay via colorimetric method and DCFDA via fluorimetric and flow cytometry methods...but I am getting very fluctuating results and especially in case of flow cytometry, i am even finding it hard to set the x and y axes of the plots; and the voltage settings...
I am just curious to know what I am doing wrong or what i can do to get a proper reading...if there are any helpful protocols