i have to analyze the expression of micro rnas in cancer samples.

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raihana
raihana's picture
i have to analyze the expression of micro rnas in cancer samples.

 i have extracted total rna using trizol reagent. can i use this total rna as such for analyzing expression of  micrornas or should i purify the microrna fraction from total rna.

which method should i use for mirna purication.

thank you