i have to analyze the expression of micro rnas in cancer samples.

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raihana
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i have to analyze the expression of micro rnas in cancer samples.

 i have extracted total rna using trizol reagent. can i use this total rna as such for analyzing expression of  micrornas or should i purify the microrna fraction from total rna.

which method should i use for mirna purication.

thank you

g a
g a's picture
Hi Raihana

Hi Raihana

You can check Qiagen's miRNA easy mini kit for puriication of microRNA.