Does anyone know of an electroporation device that uses the smallest volume for transfecting mammalian cells?
The reagents i am transfecting are extremely expensive and the smallest volume so that i can get the optimal local concentration for transfection is what i am looking for.
I am trailing the amaxa nuleofector at the moment, but the lowest volume that I have succeeded with is 50ul and it is very particular about its cuvettes.
I have another device that delivers 5ul of solution onto about a 2mm square area in the center of a 3.5mm dish. It is very efficient but very few cells see the pulse.
If there is a cuvette out there that uses 5-10ul of solution and is suitable for mammalian cell-line transfection I'd love to know about it (and the device to power it of course!)
I don't know of one that uses such a small volume. Amaxa's new 96 well format (add on to the current Nucleofector) will use a 40ul/well volume. That's a bit better, but nowhere near the 5-10ul volume. Since I haven't tried the 96 well system, I don't know if you can "fudge" the volumes down a bit. We'll have to find out later this year or early next year when it is released.
I would contact BTX. They make electroporation devices and have been since the 70's.frasermoss wrote:
already talking with them. nothing on the market to fit my needs right now
Try the microporator from Digital-bio technology. It uses a modifed pipette tip as the electrode. The sample volume is 10ul (and they have a 100ul option).
We get great transefections using it on Jurkats and DRG neurons. They have a cell database on www.microporator.com.
frasermoss wrote: