Very low volume electroporation devices

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Fraser Moss
Fraser Moss's picture
Very low volume electroporation devices

Does anyone know of an electroporation device that uses the smallest volume for transfecting mammalian cells?

The reagents i am transfecting are extremely expensive and the smallest volume so that i can get the optimal local concentration for transfection is what i am looking for.

I am trailing the amaxa nuleofector at the moment, but the lowest volume that I have succeeded with is 50ul and it is very particular about its cuvettes.

I have another device that delivers 5ul of solution onto about a 2mm square area in the center of a 3.5mm dish. It is very efficient but very few cells see the pulse.

If there is a cuvette out there that uses 5-10ul of solution and is suitable for mammalian cell-line transfection I'd love to know about it (and the device to power it of course!)

alfred
alfred's picture
I don't know of one that uses

I don't know of one that uses such a small volume. Amaxa's new 96 well format (add on to the current Nucleofector) will use a 40ul/well volume. That's a bit better, but nowhere near the 5-10ul volume. Since I haven't tried the 96 well system, I don't know if you can "fudge" the volumes down a bit. We'll have to find out later this year or early next year when it is released.

marcia (not verified)
Guest's picture
I would contact BTX. They

I would contact BTX. They make electroporation devices and have been since the 70's.frasermoss wrote:

Does anyone know of an electroporation device that uses the smallest volume for transfecting mammalian cells?

The reagents i am transfecting are extremely expensive and the smallest volume so that i can get the optimal local concentration for transfection is what i am looking for.

I am trailing the amaxa nuleofector at the moment, but the lowest volume that I have succeeded with is 50ul and it is very particular about its cuvettes.

I have another device that delivers 5ul of solution onto about a 2mm square area in the center of a 3.5mm dish. It is very efficient but very few cells see the pulse.

If there is a cuvette out there that uses 5-10ul of solution and is suitable for mammalian cell-line transfection I'd love to know about it (and the device to power it of course!)

Fraser Moss
Fraser Moss's picture
already talking with them.

already talking with them. nothing on the market to fit my needs right now

mol
mol's picture
Try the microporator from

Try the microporator from Digital-bio technology. It uses a modifed pipette tip as the electrode. The sample volume is 10ul (and they have a 100ul option).
We get great transefections using it on Jurkats and DRG neurons. They have a cell database on www.microporator.com.

frasermoss wrote:

Does anyone know of an electroporation device that uses the smallest volume for transfecting mammalian cells?

The reagents i am transfecting are extremely expensive and the smallest volume so that i can get the optimal local concentration for transfection is what i am looking for.

I am trailing the amaxa nuleofector at the moment, but the lowest volume that I have succeeded with is 50ul and it is very particular about its cuvettes.

I have another device that delivers 5ul of solution onto about a 2mm square area in the center of a 3.5mm dish. It is very efficient but very few cells see the pulse.

If there is a cuvette out there that uses 5-10ul of solution and is suitable for mammalian cell-line transfection I'd love to know about it (and the device to power it of course!)