I am new to here, and I am now trying to stain for T-bet or Foxp3 in human dendritic cells after transduction.
I used the eBioscience Foxp3 intracellular staining kit (the buffer set); and I tried different perm/fix and staining time.
I still cannot get the stainng work, there is only once that I see a 50% postitive events, there dendritic cells are transduced with Ad.T-bet, or Ad.Foxp3, normally there should be around 50% T-bet positive cells after transduction, however, I can only get around 3%-10% positive cells, which is not reflecting true efficiency of transduction.
Anyone has experience on Intracellular staining for nucleus protein in human DC? Any suggestion?
Thank you very much!