I use a BD LSR II for multicolor flow. I am using the 355nm laser for DAPI Live/Dead, but that has detector overlap issues if I'm also using the 405nm for Pacific Blue. As I understand it, Hoechst has the same problem.
I also use the 488nm for FITC, PE, PerCP-Cy5.5.
I also use the 633nm for Alexa Fluor 700, and APC-Cy7.
So, does anyone out there make a good live/dead discriminator that falls in an uncovered detector range, perhaps around where PE-Texas Red or APC or PE-Cy7, or Pacific Orande or AmCyan fall? Those are my only flexible detectors right now.
Live/Dead discriminator for flow