During differentiation my cells start detaching from the culture dishes. When fresh stocks are thawed and plated die. I am suspecting contamination with mycoplasma. Is my doubt valid? Could there be a possible contamination in my culture? I am going to have them tested for a possible contamination, but if anyone has experienced similar problems please provide your comments.
Very frustrating as one has to wait for a week to complete the differentiation. While when cells die, each time you thaw them amounts for months of wasted eforts. Has anyone has had similar problems? if yes, what solutions were tried? Many thanks