We are going to buy an automated cell counter.
Can anybody give some guidance about which one is the best and in your lab you are using Which one?
that's the one most labs are using in our institute.
But, there is some limitation of automatic cell counters, eg. won't distinguish cell clusters from a single cell. so try to trypsinize as good as you can and try to do the manual count once the readouts from the automatic one goes far off from your expectation.
We we're using ViCell from Beckman. I only used it a dozen of times and it worked very well. As Kangmu noted, clusters can be a problem but depending on the settings you have, it can figure out small clusters of 3-4 cells. I haven't tried any other brand so I can't really compare...
We use invitrogen countess machine and is very satisfied with the performance. I consider automated counter as essential to cell culture lab especially if you have to be specific like the no of cells required for experiements,harvesting at certain % confluencey and so on.
We tried a 'pipette' style counter from Millipore and found that it was wildly inaccurate. I think that unless you are counting thousands of cells for a growth promotion test, diluting and counting manually is the best way.
Thanks for the reply
I come across some other cell counter that is
"ADAM " Automated Cell Counter and for Image capturing "Juli"
Please give your opinion about this two things
buy any cell counter no problem ,buy double chamber price for chnese machines in india are about 3.0 lac
and for othe machines are more than 4.0 lac
all cell counters are good .check for number of installations in your area and about service ,use original reagents for long life of machine
We have the invitrogen countess machine and I don't like it. It rounds up to the nearest 100,000 and repeated measures give large variability. Also, consumables are really expensive. I stick to the Levy chamber.
I also have experienced variability within a range especially for viability of cells.This also depends on the accuracy of the micropipette used to dispense the 10uL of the dye and cells.I take special attention to ensure that only 10uL of the dye is added to the cells.After taking the dye I touch the tip on the side of the dye tube to remove any dye sticking to outside of the tip.
Hope this might be of some help in getting better results with countess machine.
we have an automated cell counter from ORFLO. The product name is MOXI-z which takes just takes 8 sec to measure cell size, cell count and cell viability its performing brilliantlyin US. The product is US make and it doesnot need any staining procedures.
for details visit our website www.jhindia.com
we have brought sceptor
which is automated cell counter it is very good easy to use and fast . but it would be difficult find percentage of live and dead from that
How are you, if you want to buy Automated cell counter means, contact - Analyze Biotech Company, india. for more detail, www.analyzebio.com / www.biotralc.com / www.analyzeimage.in .
i am using that company cell count for more than 2 years, service support excelent. the prices is little bit low than others company , with best quality.
hope the product name HEMOTX
Check out the NC-200. Very easy to use (2 step process); The most precise instrument out there.http://www.chemometec.com