I am working on APC 10.1 cell lines. there are small black dots in the culture. The cells are healthy, the cell morphology is normal. And there is absence of bacterial and fungal contamination.
Please, suggest what these black dots are ??????
the other thing is the cell line is very sticky and cell disaggregation is a problem. I am using trypsin for this purpose, but the cells dont detach after normal 2-5 min. There are sheets/ flakes of cells in media after tryp. I am interested in proteomics, so i am worried about prolonged use of trypsin then normal std time.
Any suggestions or comments are very welcome !!
Thanks & regards
Black dots in cell culture