I need some help. My P19 cells detach from the surface during muscle differentiation with 1% DMSO in a 14 days protocol.
I tried some things to resolve this question such as:
1. Changing my P19 cell line
2. Work with new reagent (but not human apo-transferrin that was maybe too old....)
3. Reducing the number of cells by bottle in order to avoid medium acidification.
Does anybody know what the problem is here?
Thank you very much.