Problems growing Hep-G2 cells

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nanomedicine's picture
Problems growing Hep-G2 cells

Recently i started to work with Hep-G2 cell line. I am following the ATCC protocol and medium for culturing the cells. Normally they grow nicely. But my problem is that they are not becoming 100% percent cunfluent. After 85% of confluency they started to grow on each other. Is it possible to have 100% confluent monolayer of this cell line? I need help desperately..