I need to study effect of 50 uM Sirtinol in neuronal cels. but when i add Sirtinol( stock 10mM in DMSO ; solubility: 24mg/ml or 100mM in DMSO) to MEM medium, it precipitates out.
Sirtinol added from stock: 7.5 uL in 1500uL MEM when it precipitates.
I dont have concentrated solution of Sirtinol. 10mM is the only stock I have. I m not wishing to dilute the stock with DMSO as i dont want more DMSO to be added to cells.
Is SIrtinol incompatible with MEM? HAs anybody worked with Sirtinol in DMSO in cell culture? Share your experience so that i can solve my problem.
Thanking you all in advance. Your participation is highly appreciated.