Cryopreservation of plasma

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moleculargeek21
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Cryopreservation of plasma

Hey all!

What sort of cryopreservation media will i need to consider adding to plasma isolated from whole blood to be stored at -80?

g a
g a's picture
Dear moleculargeek 21

Dear moleculargeek 21

In general plasma is stored directly at -80 C without adding any additional preservatives.
However for some specific purposes plasma is added along with a few preservatives in particular
1) formic acid to acidify the plasma.
2) Protease inhibitors (although plasma itself has number of protease inhibitors).

What is your downstream application ?

moleculargeek21
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Thanks for replying,

Thanks for replying,

I work for a blood and tissue repository.  I receive whole blood and remove plasma and buffy coat and catalogue these samples in a computer system.  Researchers throughout the campus contact us for frozen tissue specimens, DNA and RNA isolations from buffy coat or tissue, or plasma.  The downstream applications are up to the researchers.