cDNA cloning

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supaboy2925's picture
cDNA cloning

How do you clone a cDNA for a protein that is highly over expressed in cancer (colon cancer for an example)? Including an experiment to demonstrate you truly have cloned a correct DNA.

1. Design an experiment that detects every tissue is human body that expresses the cDNA you cloned in the question above.

2. Describe how you would clone and sequence the promoter responsible for the tissue-specific expression of the cDNA.

3. How could you use PCR to attach the promoter you indentified in #2 to a GFP cDNA so that GFP would be expressed in the same tissue as the original cDNA you cloned above?