I was wondeirng if anyone had the formula for poulation doubling time for cells. I have the initial and final number of cells as well as the time.
The equation I have is from "Basic cell culture : a practical approach / edited by J.M. Davis":
(1) Log (final cell number) - Log (initial cell number) x 3.32
(2) Divide by time difference (T2-T1)
(3) Get the inverse (1/n) of this value
The answer you obtain is the Population Doubling Time expressed as X hours per doubling.
If you have more data (more than two time points), you need to use an exponential curve fitting method.
You can do this with an online doubling time calculator at :
Calculating doubling time from just two cell counts is not good idea. It is better if you have data from 3 different densities and at least 4 time points for each density. Take average of all get more practical and accurate doubling time.
can u tell me, Is the doubling time would be calculated on between two passages or more