doubling time

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vserra04b
vserra04b's picture
doubling time

Hello,

I was wondeirng if anyone had the formula for poulation doubling time for cells. I have the initial and final number of cells as well as the time.

Thanks

RockyDoc
RockyDoc's picture
Hello,

Hello,

The equation I have is from "Basic cell culture : a practical approach / edited by J.M. Davis":

(1) Log (final cell number) - Log (initial cell number) x 3.32
(2) Divide by time difference (T2-T1)
(3) Get the inverse (1/n) of this value
The answer you obtain is the Population Doubling Time expressed as X hours per doubling.

snoopline
snoopline's picture
If you have more data (more

If you have more data (more than two time points), you need to use an exponential curve fitting method.
You can do this with an online doubling time calculator at :

www.doubling-time.com/compute.php?lang=en

Shubhangi
Shubhangi's picture
Calculating doubling time

Calculating doubling time from just two cell counts is not good idea. It is better if you have data from 3 different densities and at least 4 time points for each density. Take average of all get more practical and accurate doubling time.

v1tanwar
v1tanwar's picture
hello,

hello,

can u tell me, Is the doubling time would be calculated on between two passages or more