i dont know where to start

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duckydude1
duckydude1's picture
i dont know where to start

well im a freshman in highschool and i want to start learning about tissue culture but i dont know how so if you know anything that would help me plz say thanks ahead of time

marcus muench
marcus muench's picture
You need to start knocking on

You need to start knocking on some doors. Identify places around were you live were you may be able to do a summer internship. Then contact the people there and ask if you can volunteer to help out in the lab. (maybe you can get paid a little).

Your a bit young, so don't be discourged, because you have time. Just keep at it.

Fraser Moss
Fraser Moss's picture
What City are you in

What City are you in duckydude1?

Maybe SS users can come up with a list of potential places where you could apply for an internship.

Alternatively, some city colleges run technical courses in biology that provide vocational training in techniques like tissue culture. Maybe you could investigate your local city college to see if you are lucky enough that they run such a course?

duckydude1
duckydude1's picture
well i live in midland texas

well i live in midland texas so if you think of any where let me know and ill check the local college and try some other places thanks for the help.

ryan_m
ryan_m's picture
Hi duckydude.

Hi duckydude.
If your plan is to find a supervisor who will help you "attach something to your spine and attach that to your brain through nerves so you could actually use it like you use the rest of your body" as you describe in one of your other posts, you need not bother. Otherwise, good luck.

Ryan

samm
samm's picture
Hey Ryan - give the chap a

Hey Ryan - give the chap a break; and Duckydude, you really do need a break - from Hollywood! Have you had any luck with your search? You can also contact your high school board - many university outreach programs get till there (I think Tx A&M has one).

duckydude1
duckydude1's picture
ryan: my plan originally

ryan: my plan originally started as that but it has changed and now its not nearly the same except for the fact that it involves birds but i understand where your coming from. And Samm i will try and talk to the highschool board i hadn't thought of that so thank you and my idea didnt get started from hollywood its from a book then expanded off of a doctors work

tamtam
tamtam's picture
Hi all

Hi all
I have worked with  HEPG2-GFP cells for a while now, but I always find it difficult to get monolayer when plated. There  are almost always clumps of cells. I need a well spread mono layer for my work especially when nuclei count is the endpoint of interest.
How can I get my cells (HEPG2-GFP) spread out without clumps in a 384 well plate?

vanshita
vanshita's picture
HELLO TAMTAM,

HELLO TAMTAM,
TO AVOID CLUMPING , DO NOT SHAKE YOUR FLASK WHEN YOU DO PLATING OR EITHER DISPERSING CELLS VIA TRYPSINIZATION.

Abhishek Singh
Abhishek Singh's picture
dear tamtam

dear tamtam
first decide cell density to be plated because higher cell density with high serum conc will give  clumps.
second you can follow trypsinization formaking single cell suspension.
and also consider media compatibility for proper growth of these cells