PLATELET LYASTE FROM CORD BLOOD

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PLATELET LYASTE FROM CORD BLOOD

Hi,
I'm trying to obtain platelet lysate (PL) from umbilical cord blood (UCB). But, I found that there is more variations about PL from peripheral blood. I'll be appreciated, if anyone send me an optimized protocol about PL preparation from UCB with the maximum platelet count (best recovery) & more leukocyte depletion.
Best Regards

g a
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Hi Alireza 1358

Hi Alireza 1358
check the following paper entitled
Technical methods. A simple method for the isolation of blood platelets.
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=477839