I would appreciate if someone could help me in my protocol for apoptosis detection with monoclonal antibody.
I am working with cell culture of HeLa and I want to detect apoptosis using a monoclonal antibody specific for ssDNA (MAB3299), and Hoechst is the nuclear counterstain.
I am working with the dilution of 1:50 and 1:100 for MAB3299. I didn't get any results yet.
Does somebody know the best protocol for this antibody in experiment using adherent cells?