Hi I have rat periodontal cells growing in 24 well plates I think they will be confluent in a day or two and after that I have to induce apoptosis with camptothecin. I have never measured apoptosis in cells. If you could tell me how to go about with it, it would be great. I will be usung a concentration of 4 micromoles for 24 hours. I also want to know what I need to check under the microscope. I hope this is not a very silly question but I am basically new with research and I think you all are doing a good job and can help me. Thank you.