Cell Proliferation inhibitors

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pog
pog's picture
Cell Proliferation inhibitors

 Hi everybody!!, i'm doing angiogenic assays to test migration defects in my cells and between controls and ko cells the proliferation ratio is very different, i know that there are some inhibitors to block the proliferation and test the migration in 3D gels and transwell inserts but i can't find a good one. Please help me!!!

R Bishop
R Bishop's picture
Hi poq,

Hi poq,
Topic moved to Cell Proliferation Forum. hope you get some answers.

Rb

Guy Sovak
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Try to read this paper :http:
pog
pog's picture
guy wrote:Try to read this

guy wrote:

Try to read this paper :
http://circres.ahajournals.org/cgi/content/abstract/47/4/578

i did it, i have to use heparin in my media, but the proliferation ratio remains different. I know that there are a drug just to add in the media, but i can't find it again.

Shubhangi
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Hi

Hi
The simplest drug used by many researchers is Actinomycin D. It binds to DNA and stops replication. Cells will not divide but remain viable.

A Timmer
A Timmer's picture
You have to be careful using

You have to be careful using actinomycin D as it is a known inducer of apoptosis. Apoptosis can be induced with 1 ug/ml and will usually start to occur between 12-24 hours after actinomycin D treatment.

Shampa
Shampa's picture
Timmer is right as

Timmer is right as Actinomycin is avoided as its highly toxic and causes damage to genetic material. But still it is used in many labs as it is a useful tool in determining apoptosis and distinguishing between dead cells and live ones. (http://www.fermentek.co.il/7AAD.htm)

You can see more details:
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/datasheet6/a1410dat.Par.0001.File.tmp/a1410dat.pdf
http://www.ias.ac.in/jarch/jbiosci/20/151-156.pdf

R Bishop
R Bishop's picture
Poq,

Poq,
What concentration of heparin are you using?  Heparin competes for growth factors required fro angiogenesis (FGF2, etc).  Are you adding serum as well?
 
Rus

heehawmcduff
heehawmcduff's picture
Doxycycline is also an

Doxycycline is also an effective inhibitor of cell proliferation but unfortunately at high doses you also run into the same problem i.e. induction of apoptosis.  I wonder if a low dose treatment could accomplish what you are looking for.
Regards

kranthi
kranthi's picture
Hi,

Hi,
can u tell me the simple procedure to find out  DNA binding activity of anticancer drugs