cell viability

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haleema
haleema's picture
cell viability

 what for DMSO is added in MTT assay??????

heehawmcduff
heehawmcduff's picture
Hi,

Hi,

The DMSO is added to solubilise the insoluble formazan product that is generated by the cellular reduction of the MTT salt.  This then gives the ability to read the absorbance of the solution using a spectrophotometer.

Best wishes

haleema
haleema's picture
 hi

 hi
thanks for ur help...sorry to bother you again....do you have any protocol(manual) related to study the proteasomal activity in cancer cell line other than flow cytometry analysis???
haleema