I am doing Hoechst stain to detect apoptosis in my cells. Apoptotic cells have condensed chromatin which would be stained brighter than that of normal healthy cells. However, when cells undergo mitosis, their chromatin also condensed, So how do we differentiate the condensation and more brightly stained chromatin between apoptotic or mitotic cells? Flow cytometry assays also show that about half of the cell population are in G2/M arrest. so, in this case, is Hoechst staining a good way to detect apoptosis?