my research is on hela cell under differents condition of stress.
i have to induice ERS(?endoplasmic reticulum stress) by different drug like cisplatin ,H2O2,and sodium dithionite.
I'll use westen blot for detecting ERS
and my problem is how can i know the appropriate concentration,treatment duration on my cells for each of these drug.
please i realy need help
i m looking for appropriate doses of drug