MTT assay

3 posts / 0 new
Last post
Hasan
Hasan's picture
MTT assay

Salam,

I am trying to do MTT assay for group of Flavonoids to study its Antiprolifirative effect aganist colorectal cancer cell lines Caco-2 and HRT-18, I have a problems when I repeat the MTT assay for the same flavonoids and for the same cell lines in the same conditions, the results are not consistent there is no repeatability, where is the problems.
 I make a serial dilution of flavonoids in 96 well plates then cells added 10 1x104 /well, incubated for 24, 48 and 72 hrs.
including controls and vehichels DMSO & ethanol.

g a
g a's picture
 Hi Hasan

 Hi Hasan

Did you perform the cell count for all wells and performed the assay? Confluency of cells or variation of cell numbers may be a reason for it.

Hasan
Hasan's picture
Thank u. for replay, I'll try

Thank u. for replay, I'll try to do my best to adjust cells no. in each well.