Hi, I am planning on stimulating T cells with plate bound anti-CD3 and anti-CD28 I know I want to use a concentration of 1ug/ml but I'm having issues finding a protocol to do this. What do I use as a buffer for coating? what do I wash with? how long should I incubate the plate for? and at what temp?
I'm sure that lack of protocols is due to its common use in labs and therefore no need to publish it but my lab has previously used the beads rather than coated plates and therefore I have no source of this protocol near by.
any info would be a huge help, thanks