Hi i want to determine the cytotoxicty of calcitriol for my sw480 cell line, so i will use wst-1 kit. Here is my question:
Consider that i find IC50 value of calcitriol 0,000001 mM with 36 hours incubation. Is it possible to use higher concentraion and lower the incubation time? Which one is impornant? Longer incubation time or higher agent concentraion?. In my opinon longer incubation time should be important because cells then can metabolise the agent.
Thanks a lot!!