Botulinum toxin A in MCF-7

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nlamere's picture
Botulinum toxin A in MCF-7


has anybody had used the botulinum toxin A (BoNT/A) to block exocytosys in MCF-7 or epithelial cells (like HeLa)? ... I have been searching an answer on pubmed and could not find anything very satisfaying...

Thank you

Fraser Moss
Fraser Moss's picture
Nothing on breast cancer cell

Nothing on breast cancer cell lines, but if you search
"BoNT/A SNAP-25 cancer" then you get the following hits

1: Dong M, Tepp WH, Johnson EA, Chapman ER.
Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in
living cells.
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14701-6. Epub 2004 Oct 1.
PMID: 15465919 [PubMed - indexed for MEDLINE]

2: Yowler BC, Kensinger RD, Schengrund CL.
Botulinum neurotoxin A activity is dependent upon the presence of specific
gangliosides in neuroblastoma cells expressing synaptotagmin I.
J Biol Chem. 2002 Sep 6;277(36):32815-9. Epub 2002 Jun 27.
PMID: 12089155 [PubMed - indexed for MEDLINE]

3: Purkiss JR, Friis LM, Doward S, Quinn CP.
Clostridium botulinum neurotoxins act with a wide range of potencies on SH-SY5Y
human neuroblastoma cells.
Neurotoxicology. 2001 Aug;22(4):447-53.
PMID: 11577803 [PubMed - indexed for MEDLINE]

4: Huang X, Kang YH, Pasyk EA, Sheu L, Wheeler MB, Trimble WS, Salapatek A,
Gaisano HY.
Ca(2+) influx and cAMP elevation overcame botulinum toxin A but not tetanus toxin
inhibition of insulin exocytosis.
Am J Physiol Cell Physiol. 2001 Sep;281(3):C740-50.
PMID: 11502551 [PubMed - indexed for MEDLINE]

5: Huang X, Wheeler MB, Kang YH, Sheu L, Lukacs GL, Trimble WS, Gaisano HY.
Truncated SNAP-25 (1-197), like botulinum neurotoxin A, can inhibit insulin
secretion from HIT-T15 insulinoma cells.
Mol Endocrinol. 1998 Jul;12(7):1060-70.
PMID: 9658409 [PubMed - indexed for MEDLINE]