I need to lyse mammalian cells (Hela) to release GFP so that I can measure fluorescence on a microplate reader. What is the best method to lyse the cells? The paper I have uses freeze/thaw but does not mention buffer, any suggestions? Also, do I need protease inhibitors if I am just going to lysis the cells, read the fluorescence, then toss the plate?
I have never worked with proteins before so any help would be greatly appreciated.