Hi. western blot is a new technique for me as my work usually revolves around genomics microarrray. So, my lack of experience makes my work a bit difficult.
1) May I know what type of antibody needs to be used for primary and secondary? My guess is, if my primary antibody is from a mouse, so my secondary antibody should be from mouse source as well.
2) Is it advisable to to re-use the same mebrane for other antibody for up to three times?
3)If I am to set up a knockdown experiment, I need both protein and RNA. Is there any particular reason that I may need a bigger dish (i.e. P90) for protein and smaller (i.e. P60) dish for RNA?