I am here with a query.. I want to find whether apoptosis is taking place due to the agent i use on the meatazoan species. My problem is that i am not able to isolate DNA from it, and so, I need some way to prove apoptosis is taking place. I tried some stains, but they could not so easily penetrate the cuticular barrier for the parasite and hence cudnt differentiate live, apoptotic or necrotic cells.[i]
SO, can any body please suggest me with a solution???