I have a bacterial species that very effectively induces apoptosis of macrophages in vitro (60-70% in 4 hours). I also have a mutant bacteria lacking the gene responsible for the induction of apoptosis.
I am looking for a method to quantite in vivo apoptosis when the bacteria has been injected into mice. Does anyone have any experience with injecting an agent into mice and then assessing apoptosis?
Thanks very much!